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KMID : 0380020150300060283
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Korean Journal of Biotechnology and Bioengineering
2015 Volume.30 No. 6 p.283 ~ p.290
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Optimization of Cellulase Production from Paenibacillus jamilae BRC 15-1
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Cha Young-Lok
Yoon Young-Mi
Yoon Ha-Yan
Kim Jung-Kon
Yang Ji-Young
Na Han-Beur
Ahn Jong-Woong
Moon Youn-Ho
Choi In-Hoo
Yu Kyeong-Dan
Lee Ji-Eun
An Gi-Hong
Lee Kyeong-Bo
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Abstract
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In this study was selected the cellulolytic microorganism and investigated optimum condition of cellulase production for the cellulosic bioethanol production. A bacterial strain Paenibacillus jamilae BRC15-1, was isolated from soil of domestic reclaimed land. For optimizing cellulase production from the selected strain, various culture parameters were investigated such as culture medium, pH (pH 4~10), temperature (25~50°C) and culture time (2~72 h). As a result, P. jamilae BRC15-1 efficiently produced cellulase from cellulosic biomass under following conditions: 24 h of culture time (pH 7, 40°C) in manufactured media of CMC (carboxymethyl cellulose) with peptone. Optimum saccharifying condition of crude enzyme produced from P. jamilae BRC15-1 was identified on pH 6 and 40°C of reaction temperature, respectively. This crude enzyme from P. jamilae BRC15-1 was used for saccharification of pretreated sweet sorghum (Sorghum bicolor var. dulciusculum Ohwi) bagasse under the optimal condition. Finally, pretreated sweet sorghum bagasse including 0.1 g of glucan was saccharified by crude enzyme of P. jamilae BRC15-1 into 2.75 mg glucose, 0.79 mg xylose and 1.12 mg arabinose.
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KEYWORD
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Cellulase, Bioethanol, Saccharification, Paenibacillus sp., Sweet sorghum bagasse
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